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What are different methods of sterilization?

What are different methods of sterilization?

Sterilization methods

Sterilization is the process of killing or eliminating all forms of microbial life, including bacteria, viruses, fungi, and spores. Here are some common methods of sterilization:

  • Autoclaving: It is a process of sterilization that involves subjecting items to high-pressure saturated steam. The autoclave is a sealed device (similar to a pressure cooker) that kills microorganisms using saturated steam under pressure . Autoclaves are commonly used in medical and laboratory settings to sterilize instruments, glassware, and other materials.
  • Chemical sterilization: It is a process of sterilization that uses chemicals such as ethylene oxide or hydrogen peroxide gas to kill microorganisms. It is commonly used in healthcare settings to sterilize heat-sensitive equipment.

  • Radiation sterilization: It is a process of sterilization that uses ionizing radiation (such as gamma rays or X-rays) or non-ionizing radiation (such as ultraviolet light) to kill microorganisms. This method is commonly used to sterilize medical equipment, food products, and other materials.

  • Filtration: It is a process of sterilization that uses filters to remove bacteria and other microorganisms from a liquid or gas. This method is commonly used to sterilize liquids such as intravenous fluids and vaccines.

  • Dry heat sterilization: It is a process of sterilization that uses dry heat to kill microorganisms. This method is commonly used to sterilize glassware, metal instruments, and other materials that can withstand high temperatures 
  •  Heat is carried from its source to load by radiation, convention and to a small extent by conduction

  • Boiling: It is a simple and effect method of sterilization that involves boiling items in water for a set period of time to kill microorganisms. It is commonly used in home settings to sterilize baby bottles, kitchen utensils, and other items.

  • What are different methods of sterilization?

It is important to note that not all methods of sterilization are suitable for all types of materials or microorganisms. The choice of sterilization method depends on the type of material being sterilized, the type of microorganisms present, and the intended use of the sterilized material.


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